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The separation of biomolecules using capillary electrochromatography

✍ Scribed by Hongjing Fu; Xiaodong Huang; Wenhai Jin; Hanfa Zou


Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
96 KB
Volume
14
Category
Article
ISSN
0958-1669

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✦ Synopsis


The unique properties of capillary electrochromatography such as high performance, high selectivity, minimum consumption of both reagents and samples, and good compatibility with mass spectrometry make this technique an attractive one for the analysis of biomolecules including peptides, proteins, carbohydrates, nucleosides and nucleotides. Irreversible adsorption between the biomolecules and the charged packing surface leads to a lack of reproducibility and serious peak tailing, so various approaches have been taken to overcome this and to improve the technique for future challenges.


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