The Role of Isotope Dilution Mass Spectrometry in the Development of Tandem Mass Spectrometry for Quantitative Organic Analysis

✍ Scribed by Kenneth S. Webb; David Carter

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 86 KB
• Volume: 11
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19970131)11:2<155::aid-rcm728>3.0.co;2-d

✦ Synopsis

Tandem mass spectrometry (MS/MS) has the significant advantage of improving selectivity and overcoming interferences present in conventional mass spectrometry. The quantitative use of MS/MS is discussed with reference to sources of instrumental error and ways to minimize such errors. Key parameters are collision energy and collision gas pressure. Instrumental stability and tuning are also very important. Even with all parameters optimized reproducibility is poor but can be significantly improved by using MS/MS in combination with isotope dilution mass spectrometry (IDMS). Comparative measurements using MS/MS, a combination of IDMS and MS/MS (IDMS/MS), and IDMS alone on a range of reference materials showed that the use of IDMS/MS improved the reproducibility of quantitative measurements by almost an order of magnitude. Although IDMS/MS is inferior to IDMS alone it is ideally suited to those analyses where the tandem mode can be used to overcome interference problems present in conventional IDMS.