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The Role of First and Second Shell Interactions in Phosphate Binding Proteins

✍ Scribed by Gruber, Mathias; Junker, Caroline; Greisen, Per; Hélix-Nielsen, Claus


Book ID
122323753
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
37 KB
Volume
104
Category
Article
ISSN
0006-3495

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The purification of a phosphate-binding protein (PiBPz) by immunoadsorption is described. The entire anti phosphate-binding protein 2 antibodies as well as the Fab fragments obtained from these antibodies inhibit P~ uptake by whole cells. The inhibition is a mixed type of inhibition (V~, and Km are