Phosphate uptake in the yeastCandida tropicalis: purification of phosphate-binding protein and investigations about its role in phosphate uptake

✍ Scribed by Robert Jeanjean; Sylvie Bedu; José Rocca-Serra; Christian Foucault

• Book ID: 104764827
• Publisher: Springer
• Year: 1984
• Tongue: English
• Weight: 607 KB
• Volume: 137
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00414546

✦ Synopsis

The purification of a phosphate-binding protein (PiBPz) by immunoadsorption is described. The entire anti phosphate-binding protein 2 antibodies as well as the Fab fragments obtained from these antibodies inhibit P~ uptake by whole cells. The inhibition is a mixed type of inhibition (V~, and Km are affected). These results should be regarded as a possible involvement of phosphate-binding protein 2 in P~ uptake. The binding of ~ 25I-labelled fragments prepared from anti phosphate-binding protein 2 antibodies to whole cells, to shocked cells and to protoptasts has been investigated. The results confirm the release of phosphate-binding protein by osmotic shock and during protoplast formation. From these findings, a cell-wall localisation, near the cell surface of the phosphate-binding protein should be proposed.