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The role of entropy and enthalpy factors in kinetic specificity of α-chymotrypsin: Temperature dependence study of acyl-α-chymotrypsins deacylation

✍ Scribed by Karel Martinek; V.N. Dorovska; S.D. Varfolomeyev; I.V. Berezin


Book ID
115749202
Publisher
Elsevier Science
Year
1972
Weight
380 KB
Volume
271
Category
Article
ISSN
0005-2795

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The acyl-enzyme formed upon acylation of a-chymotrypsin with isatoic anhydride has been characterised by infrared spectroscopy. Acylation at pH 7 to yield the 2-aminobenzoyl-enzyme is rapid (k 5.57 Â 10 À2 s À1 ), while deacylation is much slower (k 3.7 Â 10 À5 s À1 ). The [ 13 CO]-labelled form of