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The role of elongation factors in protein synthesis rate variation in white teleost muscle

✍ Scribed by Karl Jürss; Ilse Junghahn; Ralf Bastrop

Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
620 KB
Volume
162
Category
Article
ISSN
0174-1578

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✦ Synopsis

Protein synthesis-stimulating activity was assayed in the cytosolic fraction of white muscle from teleost fish (rainbow trout, carp) and of rat liver. In vitro protein synthesis-stimulating activity in the cytosolic fraction is reduced by food deprivation. The addition of elongation factors EF1, EF2, or EF1 + EF2 compensates for the starvation-induced loss of protein synthesis-stimulating activity in trout muscle cytosol. The action of EF2 is stronger than that of EF1 in this respect. However, EF1 enhances in vitro protein synthesis-stimulating activity in rat liver cytosol more than EF2. The EF2 concentration in the cytosolic fraction of white muscle from starved trout is significantly lower than in fed specimens.

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