The role of cytokines in polymyositis

✍ Scribed by Apostolos E. Kalovidouris; Claire A. Horn; Zoya Plotkin

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 753 KB
• Volume: 37
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780370620

✦ Synopsis

Objective. To investigate the effect of interferony (IFNy) on the adhesive interactions between human peripheral blood T cells and human skeletal muscle cells at various stages of muscle cell differentiation and maturation in vitro.

Methods. Human muscle cell cultures were established from normal muscle biopsy material, using the explant technique. T cells were studied for their capacity to adhere to IFNy-treated and untreated myoblasts and myotubes. The role of intercellular adhesion molecule type 1 (ICAM-1) in cell adhesion to muscle cells was examined in blocking studies, by enzyme-linked immunosorbent assay (ELISA), and by immunohistochemical staining using monoclonal antibodies (MAb).

Results. Treatment of muscle cells (myoblasts and myotubes) with IFNy resulted in a significant dose-dependent increase in the number of adherent T cells. Adhesion of T cells to muscle cells was significantly inhibited by MAb to ICAM-1 and to lymphocyte function-associated antigen type 1, but not by MAb to HLA-DR. There was no difference in the level of T cell adhesion to IFN y-treated allogeneic versus autologous muscle cells. By ELISA and immunohistochemical ana-