The ribosomal RNA genes in synthetic tetraploids of wheat
β Scribed by O. H. Frankel; W. L. Gerlach; W. J. Peacock
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 786 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0040-5752
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We have examined the number of rRNA genes in Mycoplasma capricolum (KID) by hybridization of Bg/II-, EcoRI- and Xbal-digests of DNA to [3'-32P] 16S, 23S and 5S rRNAs according to the Southern procedure (1975). All the restriction gels gave two radioactive bands with three kinds of rRNA. Furthermore,
DNA segments carrying rRNA genes of Mycoplasma capricolum have been cloned and characterized by restriction endonuclease mapping, DNA-RNA hybridization and nucleotide sequencing. The M. capricolum genome has two sets of rRNA gene clusters, where the arrangement is in the order of (5')16S-23S-5S(3').