The relationship of the cell surface to metabolism. IV. The role of cell surface phosphatases of yeast

✍ Scribed by Rothstein, Aser ;Meier, Rebecca

• Publisher: Wiley (John Wiley & Sons)
• Year: 1949
• Tongue: English
• Weight: 827 KB
• Volume: 34
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030340107

✦ Synopsis

I t has been shown by isotope techniques (Rothstein and AIeier, '48) that a number of acid phosphatases are located on the cell surface of yeast. These phosphatases can hydrolyze such substrates as adenosine triphosphate, adenosine diphosphat e, inorganic t riphosphat e and pyrophosphat e, phenyl pliospha t e and glyceropho sphat e, with the production of inorganic orthophosphate. Although some of the properties of the enzymic reactions such as the time course of hydrolysis, the effect of substrate concentration and the effect of pH have been investigated in some detail, no information concerning the role of these enzymes in the over-all economy of the cell has been available. I n fact, very little information is available concerning specific functions of any of the phosphatases. The properties of the yeast cell surface phosphatases can be readily studied in the living cell. F o r this reason, it seemed possible that the relationship ot these particular phosphatases to other cell functions could he investigated experimentally .

Three possible relationships seemed worthy of consideration. First, the surface phosphatases may play a direct role This paper is based on work performed under contract with the United States Atomic Energy Commission at the University of Rochester Atomic Energy Project, Rochester, New York.