𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The relationship between multi-drug resistance and resistance to natural-killer-cell and lymphokine-activated killer-cell lysis in human leukemic cell lines

✍ Scribed by Robin S. Treichel; Sarah Olken

Publisher
John Wiley and Sons
Year
1992
Tongue
French
Weight
627 KB
Volume
50
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Results concerning a possible link between susceptibility to natural‐cell‐mediated immune cytolysis and the multi‐drug resistance (MDR) phenotype are conflicting. We evaluated in human acute lymphocytic leukemia the relationship between acquired drug resistance and susceptibility to cytolysis mediated by endogenous, interferon‐activated, and interleukin‐2‐ activated natural cytotoxic celts. Eight human leukemia drug‐ resistant/sensitive cell line pairs were evaluated; drug‐resistant sub‐lines included those selected for primary resistance to adriamycin, etoposide, teniposide, vincristine, and vinblastine. A majority of P‐glycoprotein‐positive MDR sub‐lines displayed slight but statistically significant resistance to endogenous and/or interferon‐activated natural‐killer(NK)‐cell‐mediated lysis, as compared with the drug‐sensitive parental type. P‐glycoprotein‐ negative sub‐lines displayed variable NK susceptibility; within this group, the variants selected for primary etoposide resistance were more susceptible to NK cytolysis than parental cells. Results of cold‐target‐inhibition experiments suggest that altered NK susceptibility does not arise solely from modulation of NK target recognition and adherence structures. IL2‐activated killer (LAK) cells lysed both drug‐sensitive and drug‐resistant lines. Two MDR lines selected for primary etoposide resistance displayed enhanced LAK susceptibility. In contrast, the 2 variants selected for resistance to adriamycin exhibited partial resistance to LAK‐mediated killing, which could be overcome at high effector‐to‐target ratios. Our results support the development of interleukin‐2/LAK immunotherapy for the treatment of leukemias with acquired drug resistance.

📜 SIMILAR VOLUMES

Human tumor cell lines with pleiotropic
Human tumor cell lines with pleiotropic drug resistance are efficiently killed by interleukin-2 activated killer cells and by activated monocytes
✍ P. Allavena; M. Grandi; M. D'Incalci; O. Geri; F. C. Giuliani; A. Mantovani 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 French ⚖ 371 KB

Two human cell lines, the colon carcinoma Lovo and the BME-supplemented medium with 10% FBS. 1mM glutamhe, transformed intestinal 1-407, and their variants (LOVO /Dx and 50 u/ml penicillin and 50 pg/ml streptomycin. I-407/Dx), with pleiotropic resistance to cancer chemotherapeutic drugs, were exami

Relationship between sensitivity to natu
Relationship between sensitivity to natural killer cells and MHC class-I antigen expression in colon carcinoma cell lines
✍ Hervé M. Blottière; Rahima Zennadi; Cedric Burg; Jean-Yves Douillard; Khaled Mef 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 French ⚖ 588 KB

## Abstract The sensitivity of colorectal tumors to NK‐cell‐mediated cytotoxicity and their expression of major histocompatibility complex (MHC) class‐I antigens were studied in an attempt to determine whether such antigens play a role in the susceptibility of colorectal tumors to NK‐cell lysis. In

Altered conjugate formation and altered
Altered conjugate formation and altered apoptosis of multidrug-resistant human leukemia cell line affects susceptibility to killing by activated natural killer (NK) cells
✍ Robin S. Treichel; Michael Bunuan; Nancy Hahn; Kathleen Wee 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 136 KB

## Abstract Most leukemias that exhibit P‐glycoprotein (P‐gp)‐associated multidrug resistance (MDR) exhibit reduced susceptibility to immune cytotoxicity mediated by natural killer (NK) cells. To explore this phenomenon we investigated N6/ADR, a doxorubicin‐selected, P‐gp‐positive variant of the hu

The relationship of clinical status and
The relationship of clinical status and therapeutic modality to natural killer cell activity in human breast cancer
✍ Bluma G. Brenner; Gerald Friedman; Richard G. Margolese 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 550 KB

The role of clinical status and therapeutic intervention on natural cell-mediated cytotoxicity in breast cancer was ascertained by monitoring natural killer (NK) cell activity in peripheral blood samples. Patients with localized disease on chemotherapy showed significant reductions in NK activity co