Human tumor cell lines with pleiotropic drug resistance are efficiently killed by interleukin-2 activated killer cells and by activated monocytes

✍ Scribed by P. Allavena; M. Grandi; M. D'Incalci; O. Geri; F. C. Giuliani; A. Mantovani

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: French
• Weight: 371 KB
• Volume: 40
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910400119

✦ Synopsis

Two human cell lines, the colon carcinoma Lovo and the BME-supplemented medium with 10% FBS. 1mM glutamhe, transformed intestinal 1-407, and their variants (LOVO /Dx and 50 u/ml penicillin and 50 pg/ml streptomycin.

I-407/Dx), with pleiotropic resistance to cancer chemotherapeutic drugs, were examined for their susceptibility to human Lovo/Dx was derived from a human colon adenocarcinoma Interleukin-2-activated killer cells and to activated mono-cell line (LOVO) as previously described (Grandi et cytes. These non-specific or broadly specific effector cells ex-In brief, exponentially growing LOVO Cells were passaged pressed cytotoxicity levels on PIeiotropicaIIy resistant tumor every 2 to 3 weeks in growth medium containing Dx at the cells comparable to those of the parental cell populations. concentration of 100 pglml. After 5-6 courses of treatment, This finding provides a rationale for immunological ap-surviving cells were cloned in the presence of 100 &ml Dx. proaches designed to eradicate residual tumor cells surviving The same procedure was used to select the I-407/Dx cell line and resistant to cytotoxic chemotherapy.