The relationship between DNA strand-scission and DNA synthesis inhibition in HeLa cells treated with neocarzinostatin
β Scribed by Terry A. Beerman; Irving H. Goldberg
- Book ID
- 115746544
- Publisher
- Elsevier Science
- Year
- 1977
- Weight
- 807 KB
- Volume
- 475
- Category
- Article
- ISSN
- 0005-2787
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## Abstract The effect of chlorambucil on the colonyβforming ability of HeLa cells following either treatment for 1 h or continuous treatment has been measured. Concentrations of chlorambucil which had only minimal effects on cell survival inhibited the rate of DNA but not RNA and protein synthesis
## Abstract Flow cytometry indicated that significant amounts of dsRNA were accumulated in HeLa S3 cells blocked at or near G~1~/S boundary by hydroxyurea (HU) or excess thymidine (TdR). The dsRNA/DNA ratio increased in these cells in a manner characteristic of unbalanced cell growth. In HUβtreated