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The reaction of rhodospirillum rubrum cytochrome c2 with iron hexacyanides

✍ Scribed by Fern E. Wood; Michael A. Cusanovich


Publisher
Elsevier Science
Year
1975
Weight
1004 KB
Volume
4
Category
Article
ISSN
0006-3061

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✦ Synopsis


The reaction of Rhodospirillum rubrum cytochrome c2 with the nonphysiological reactants, ferrocyanide and ferricyanide has been investigated as a function of ionic strength, temperature and pH, using both stopped-flow and temperature-jump kinetic methods. The results are consistent with a complex reaction mechanism involving the formation of two intermediate complexes. The site of electron transfer appears to be at the front of the cytochrome c2 molecule near the hem e crevice with interacton of both ferri and ferrocyanide with a positively charged region of the molecule. Comparison of the proposed electron transfer mechanism of cytochrome c2 with ferro-ferricyanide is made with the mechanism proposed based upon structural considerations.


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