The reaction of Rhodospirillum rubrum cytochrome c2 with the nonphysiological reactants, ferrocyanide and ferricyanide has been investigated as a function of ionic strength, temperature and pH, using both stopped-flow and temperature-jump kinetic methods. The results are consistent with a complex re
Preparation of cytochrome c2 from Rhodospirillum rubrum
β Scribed by D.L. Sponholtz; D.L. Brautigan; P.A. Loach; E. Margoliash
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 339 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
A simplified method is described for the preparation and purification of cytochrome cZ from Rhodspirillum rubrum growth photosynthetically under anaerobic conditions. The cytochrome c1 appeared as a single electrophoretic and chromatographic form at all stages of the preparation.
π SIMILAR VOLUMES
The effect of a number of salts on the activity of the particulate NADH-cytochrome c-reductase of Rhodospirillum rubrum was investigated. The enzyme was shown to be inhibited by the presence of these salts. With monovalent anions a relationship between the size of the anion and its capacity of inhib
The light-harvesting B880 complex of Rhodospirillum rubrum was purified by a new method which allowed recovery of 66% of the amount present in the crude solubilized extract. Electrophoretic analysis of the isolated complex, followed by either Coomassie brilliant blue or silver staining, revealed onl