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The rapid activation in vitro of the chloroplast fructose 1,6-bisphosphatase followed using a new assay procedure

✍ Scribed by Luciana Rosa

Book ID
115912346
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
359 KB
Volume
134
Category
Article
ISSN
0014-5793

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A new procedure for the purification of spinach leaf photosynthetic fructose-1,6-bisphosphatase by affinity chromatography on mercaptoethylamine-Sepharose
✍ A. PlΓ‘; A Chueca; J LΓ³pez-GorgΓ© πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 495 KB

A new purification procedure for spinach leaf fructose-l,6-bisphosphatase is proposed, which includes the use of affinity chromatography on mercaptoethylamine-Sepharose. A homogeneous preparation of the enzyme can be obtained in 48 hr, with a specific activity of 67 U/rag and a yield of 23%. The met