The qualitative separation of fatty acids by isotachophoresis

✍ Scribed by J.L. Beckers; F.M. Everaerts; W.J.M. Houtermans

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 195 KB
• Volume: 76
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97812-4

✦ Synopsis

The qualitative separation of fatty acids by isotachophoresis Fatty acids can be determined in various ways, but many methods require complicated or tedious prc-treatments.

In general, electrophoretic methods do not require pre-treatments.

One of the electrophoretic methods is isotachophoresis, an analytical method for the qualitative and quantitative analysis of ionic species"-".

The separation of anionic species has been discussed earlier2, but many of the fatty acids have about the same effective mobility and some of them are not sufficiently soluble in water. Because methanol6 can be used as a solvent in isotachophoresis and because it is a better solvent for fatty acids, the separation of fatty acids has been studied with methanol as a solvent.

An apparatus similar to that described earlier" was used. Most chemicals were obtained from Merck; not all of them were of p.a. quality, but no adverse effectswere obtained when small amounts of the ionic species were used.

Before use, the methanol (technical grade, gG % by weight) was prepared by running it through a column filled with a mixed-bed ion exchanger so as to remove ionic impurities.