The analysis of amino acids and peptides by isotachophoresis
β Scribed by Christopher J. Holloway; Vera Pingoud
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 980 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0173-0835
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π SIMILAR VOLUMES
Two peptides, one undecapeptide and one decapeptide, have been synthesized by the solid phase method. Isotachophoresis has been used as an analytical tool to guide the purification of the peptides. This technique gives qualitative as well as quantitative information about the purification progress.
A simple and highly sensitive method for quantitative amino acid and peptide analysis is described. It makes use of the reaction with [3H] dansyl chloride chromatography on polyamide plates, autoradiography at low temperature, and densitometry. This method was used for the quantitative analysis of t