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โœฆ   LIBER   โœฆ

Quantitative analysis of amino acids and peptides in the femtomolar range

โœ Scribed by S.R. Burzynski

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
887 KB
Volume
65
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple and highly sensitive method for quantitative amino acid and peptide analysis is described. It makes use of the reaction with [3H] dansyl chloride chromatography on polyamide plates, autoradiography at low temperature, and densitometry. This method was used for the quantitative analysis of the amino acids in a complete enzymic hydrolyzate of the synthetic hexapeptide H-Leu-Trp-Met-Arg-Phe-Ala-OH.

The limit of sensitivity for amino acids and small peptides is about 25 x lo-r5 mole with errors between 2 and 14% depending on the amino acid.

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