The pyridine nucleotide cycle of Salmonella typhimurium: Genetic characterization of the pncXA operon
โ Scribed by Hill-Chappell, Jean M. ;Spector, Michael P. ;Foster, John W.
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 902 KB
- Volume
- 205
- Category
- Article
- ISSN
- 0026-8925
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The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined. The gene encoded a 264 amino acid protein that was homologous to a similar protein from Escherichia coli. The sequence of an overproducer mutant allele, pepM10
It is known that cdd- S. typhimurium mutants selected for resistance to 5-fluorodeoxycytidine (FdCyd) possess no deoxycytidine kinase activity. The present study postulates that this method of screening selects double mutants defective in cytidine deaminase and in deoxycytidine kinase. To prove this