THE PURIFICATION OF THE ACETYLCHOLINE RECEPTOR FROM HUMAN MUSCLE
โ Scribed by F. Anne Stephenson; George G. Lunt; Roger Harrison
- Book ID
- 118719319
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 70 KB
- Volume
- 377
- Category
- Article
- ISSN
- 0890-6564
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We measured the concentration of acetylcholine receptor (AChR) in various normal human muscles, obtained at autopsy, in order to find a more reliable and convenient source than the amputated leg muscle from diabetic patients for the isolation of AChR. We found that human calf muscle contains t h e h
The acetylcholine receptor protein from human muscle was extracted with the non-ionic detergent Triton X-100 and purified by affinity chromatography on a-Naja toxin sepharose 4B. Further purification on Dicap-MP sepharose 4B, a choline analog compound, led to ACHR preparations with specific activiti