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Channel Blocking Properties Of Tetramethylammonium At The Human Muscle Acetylcholine Receptor

โœ Scribed by Lape, Remigijus; Colquhoun, David; Sivilotti, Lucia


Book ID
119205355
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
46 KB
Volume
96
Category
Article
ISSN
0006-3495

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The acetylcholine receptor protein from human muscle was extracted with the non-ionic detergent Triton X-100 and purified by affinity chromatography on a-Naja toxin sepharose 4B. Further purification on Dicap-MP sepharose 4B, a choline analog compound, led to ACHR preparations with specific activiti