The purification and immobilization of Penicillium notatum β-galactosidase

✍ Scribed by J. Rogalski; J. Łobarzewski

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 587 KB
• Volume: 15
• Category: Article
• ISSN: 0138-4988
• DOI: 10.1002/abio.370150211

✦ Synopsis

Penicillium notatum

No. 1 as a producer of Pgalactosidase was cultivated in a 5-1 fermenter. Various methods of protein isolation and concentration from the culture fluid were optimized. Then the conditions of P-galactosidase purification using an affinity chromatographic technique were established.

The purified enzyme was immobilized on a controlled porous glass (CPG). The optimum temperature and pH values of the native and immobilized forms of Pgalactosidase were determined as 5OoC and 30 -50 OC as well as pH 3 and pH 3-5, respectively.