The proton extrusion of growing yeast cultures as an on-line parameter in fermentation processes: Quantitative determination of growth from milligram amounts of substrate in a minimized fed-batch fermentation apparatus

✍ Scribed by Dr. Joachim Huth; Sabine Werner; Hans-Georg müller

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 450 KB
• Volume: 30
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620300706

✦ Synopsis

The proton extrusion ofgrowingyeastcultureswasdemonstrated to bea veryexact and reliable on-line parameter for the description of the growth of yeasts and substrate consumption in batch fermentation processes. Using this parameter we were able to build up a minimized fermentation apparatus (working volume about 90 ml) which permits the description of growth from substrate amounts of 5 to 50 mg quantitatively in fed-batch fermentation processes.

Using various yeast strains it was demonstrated for nitrogen-free carbon sources such as hydrocarbons and carbohydrates that I . the course of the proton extrusion indicates qualitatively whether any given carbon source can be utilized as a growth substrate or not, and that 2. the amount of proton extrusion is a reliable and reproducible quantitative parameter for growth on the tested substrate. This minimized fermentationapparatusoffersadvantagesandpossibilities to detect growth ofmicroorganisms Rom very small substrate amounts in submerged cultures.