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The proton extrusion of growing yeast cultures as an on-line parameter in fermentation processes: Ammonia assimilation and proton extrusion are correlated by an 1:1 stoichiometry in nitrogen-limited fed-batch fermentations

✍ Scribed by Dr. Joachim Huth; Sabine Werner; Hans-georg Müller

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
433 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

Inpreccdingpapers(H~.r~etuI. 1990a, b)

theprotonextrusionnH, ofgrowingyeastcultureswasshowntobean exact and reliable on-line parameter for the description of biomass production and consumption of the nitrogen (NH; ) and carbon sources quantitatively in batch and fed-batch ferincntation processcs.

In the present paper we demonstrate that the biochemical basis of this proton extrusion must be connected with the nitrogen metabolism of growing yeasts: A strong acidification was found only during growth on NH: as the source of nitrogen, while the pH-course was different for other substrates. Using NHl-limited fed-batch conditions we detected an NH:/H+ exchange ratio of exactly 1.0. The usefulness of the proton extrusion as an on-line parameter in fermentation control and its range of application are discussed.

📜 SIMILAR VOLUMES

The proton extrusion of growing yeast cu
The proton extrusion of growing yeast cultures as an on-line parameter in fermentation processes: Quantitative determination of growth from milligram amounts of substrate in a minimized fed-batch fermentation apparatus
✍ Dr. Joachim Huth; Sabine Werner; Hans-Georg müller 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 450 KB

The proton extrusion ofgrowingyeastcultureswasdemonstrated to bea veryexact and reliable on-line parameter for the description of the growth of yeasts and substrate consumption in batch fermentation processes. Using this parameter we were able to build up a minimized fermentation apparatus (working

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The proton extrusion of growing yeast cultures as an on-line parameter in fermentation processes: Determination of biomass production and substrate consumption in batch experiments with Candida maltosa EH 15 D
✍ Dr. Joachim Huth; Rosel Blasig; Sabine Werner; Hans-Georg Müller 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 449 KB

## Abstract Using the proton extrusion of growing yeast cultures (calculated from the alkali consumption required to maintain a constant pH‐value) it is possible to describe the growth of yeasts and the growth‐related substrate consumption on‐line and to do so more exactly than by determining these