The properties of trichloracetic acid-ethanol extracts of human plasma

✍ Scribed by J. S. Cameron; D. R. Boyns; R. J. Jarrett; H. Keen

• Publisher: Springer
• Year: 1966
• Tongue: English
• Weight: 502 KB
• Volume: 2
• Category: Article
• ISSN: 0012-186X
• DOI: 10.1007/bf00423015

✦ Synopsis

Bioassay, using the rat hemidiaphragm and epididymal fat pad preparations, has been performed upon the plasma and upon a T.C.A.-ethanol extract of the plasma of fourteen newly diagnosed diabetics. Immunoassay of the extracts was also carried out. Both bioassay and immunoassay of three pooled T.C:A.-ethanol extracts (Dr. W.L. As~o~) were performed. --Five of thirteen plasmas tested showed insuhn-like activity (ILA) on the hemidiaphragm, while all of fourteen tested on the fat pad showed ILA. In contrast, eleven of four~eem extracts revealed considerable amounts of ILA on the hemidiaphragm. Again, all the extracts revealed ILA upon the fat pad. Immunoreactive insulin was found in all extracts, but the levels of 'insulin' measured by this method were much lower than those measured by the fat pad assay. No extract showed significant antagonism to the effects of added insulin (1 mu/ml) on the hemidiaphragm. --Two of the pooled extracts contained very large amounts of both bioassayable and immunoreactive insulin. No one of the three, however, in our experiments, showed any significant antagonism to the effects of added insulin on the hemidiaphragm.