The propagation of s virus of mouse hepatitis in tissue culture
β Scribed by Gompels, Annette E. H. ;Niven, Janet S. F.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1953
- Tongue
- English
- Weight
- 217 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0368-3494
No coin nor oath required. For personal study only.
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A method for estimating residual infectious hepatitis A virus (HAV) after heat treatment of suspensions of the virus was devised. It made use of a readily maintained cell line (FRhK-4) in which the rate of release of HAV antigen into the tissue culture medium was directly proportional to the size of
The adenosine analog 5,6-dichloro-l-~-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. Ho
## Abstract Human diploid fibroblasts and human primary liver cell carcinoma cells (PLC/PRF/5) were infected with hepatitis A virus (HAV) adapted to growth in cell culture or derived directly from human stool. Viral antigen was expressed in PLC/PRF/5 cells 28 days after infection with cell cultureβ