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Propagation of hepatitis A virus in human embryo fibroblasts

✍ Scribed by Dr. Verena Gauss-Müller; Gert G. Friösner; Friedrich Deinhardt

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
486 KB
Volume
7
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Human diploid fibroblasts and human primary liver cell carcinoma cells (PLC/PRF/5) were infected with hepatitis A virus (HAV) adapted to growth in cell culture or derived directly from human stool. Viral antigen was expressed in PLC/PRF/5 cells 28 days after infection with cell culture‐adapted HAV, and 50 days after infection with virus from human stool. In human fibroblasts the periods until first expression of viral antigen were 90 and 210 days, respectively. During further passages of HAV in fibroblasts the time of first appearance of antigen decreased to about 28 days. Biophysical properties of HAV extracted from infected fibroblasts were comparable to those of HAV derived directly from human stool. Immunofluorescence studies showed that the antigen was located exclusively within the cytoplasm of the infected fibroblasts. Kinetics of antigen production indicated that an equilibrium between virus multiplication and cell metabolism was reached in persistently infected fibroblasts.

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