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The production of l(+) and d(−) lactic acid and volatile acids by human dental plaque and the effect of plaque buffering and acidic strength on pH

✍ Scribed by Dorothy A.M. Geddes

Book ID
115710455
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
587 KB
Volume
17
Category
Article
ISSN
0003-9969

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The analysis of picomole amounts of l(+)
The analysis of picomole amounts of l(+)- and d(−)-lactic acid in samples of dental plaque using bacterial luciferase
✍ Stanley A. Robrish; Michael A. Curtis; Sue A. Sharer; William H. Bowen 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 505 KB

I(+)-Lactic acid (5 pmol) and D(-)-lactic acid (20 pmol) were assayed by coupling the generation of NADH with the use of bacterial luciferase. The binding of NADH to r-(+)-lactic dehydrogenase made it necessary to denature the protein so that the assay with bacterial luciferase was effective. The co