✦ LIBER ✦
The analysis of picomole amounts of l(+)- and d(−)-lactic acid in samples of dental plaque using bacterial luciferase
✍ Scribed by Stanley A. Robrish; Michael A. Curtis; Sue A. Sharer; William H. Bowen
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 505 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
I(+)-Lactic acid (5 pmol) and D(-)-lactic acid (20 pmol) were assayed by coupling the generation of NADH with the use of bacterial luciferase. The binding of NADH to r-(+)-lactic dehydrogenase made it necessary to denature the protein so that the assay with bacterial luciferase was effective. The coupled luciferase assay of L(+)-lactic acid was 400 times more sensitive than the fluorometric assay. The luciferase coupled assay was used to analyze the L(+)-and N-)-lactic acid contents of small samples of dental plaque.