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The Production of 3-Ketosucrose by Agrobacterium tumefaciens in Batch Culture

✍ Scribed by Kurowski, W. Martin ;Darbyshire, John

Publisher
Blackwell Publishing
Year
2010
Tongue
English
Weight
174 KB
Volume
28
Category
Article
ISSN
0142-0356

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✦ Synopsis

Abstract

Batch cultures of Agrobacterium tumefaciens were grown in a stirred reactor holding 400 litres of a minimal medium containing 20 g of sucrose litre^βˆ’1^. 3‐Ketosucrose accumulated in the medium during the growth phase to reach a maximum concentration of 6–8 g litre^1^ at the point of sucrose exhaustion, 18–20 h after inoculation. The 3‐ketosucrose produced was free from other sugars. The yield of 3‐ketosucrose was influenced by initial aeration rate and inoculum size.

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