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Effect of sucrose on nisin production in batch and fed-batch culture by Lactococcus lactis

✍ Scribed by Wenhua Lv; Wei Cong; Zhaoling Cai

Publisher
Wiley (John Wiley & Sons)
Year
2005
Tongue
English
Weight
102 KB
Volume
80
Category
Article
ISSN
0268-2575

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✦ Synopsis

Abstract

The effects of sucrose on cell growth and nisin production by Lactococcus lactis were investigated in batch and pH feed‐back controlled fed‐batch cultures. In batch cultures, nisin titer reached its maximum, 2658 IU cm^βˆ’3^, at the initial sucrose concentration of 30 g dm^βˆ’3^. With sucrose concentrations higher than 30 g dm^βˆ’3^, nisin production decreased while the biomass was not influenced significantly. By using the pH feed‐back controlled method, residual sucrose concentration could be controlled well in fed‐batch cultures and three conditions (sucrose maintained at 2, 16, 20 g dm^βˆ’3^, respectively) were evaluated. Maintaining a low sucrose concentration at 2 g dm^βˆ’3^ during feeding favored nisin biosynthesis, and the maximum nisin titer obtained was 4961 IU cm^βˆ’3^ compared with 3370 IU cm^βˆ’3^ (16 g sucrose dm^βˆ’3^)and 3498 IU cm^βˆ’3^ (20 g sucrose dm^βˆ’3^), respectively. Copyright Β© 2005 Society of Chemical Industry

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