The process of logarithmic dilution

✍ Scribed by Edward L. Duggan; Nelson Gurll

• Publisher: Elsevier Science
• Year: 1965
• Tongue: English
• Weight: 477 KB
• Volume: 11
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(65)90016-3

✦ Synopsis

In connection with the development of a qualitative test for transforming DNA in the Bacillus subtilis system, it was recognized that the common equations for the preparation of gradients (l-4) implied that the same technique could be used to dilute the transformant DKA exponentially, prior to testing droplets of the eluent for transformation.

In this use, the activity at greatest dilution would be matched against a standard DXA at various dilutions. This amount,s to an extinction test for transformat,ion.

Tests of the dilution system will be described in this paper, for phosphate (P"'), bromcresol green, and hydrogen ion. Comparison of theory and prartice indicates that a degree of empiricism remains. The dye dilution technique proved most useful; the pH dilution test proved undependable. The theory and practical tests are described under "Results.