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The Primary Structure of Escherichia coli K12 2-Deoxyribose 5-Phosphate Aldolase : Nucleotide Sequence of the deoC Gene and the Amino Acid Sequence of the Enzyme

✍ Scribed by Poul VALENTIN-HANSEN; Finn BOËTIUS; Karin HAMMER-JESPERSEN; Ib SVENDSEN


Book ID
115121224
Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
524 KB
Volume
125
Category
Article
ISSN
1432-1327

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The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain