𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The presence of concanavalin A and canatoxin inCanavalia ensiformisDC tissue culture

✍ Scribed by Alice Sato; Grace B. S. Barcellos; Eduardo Corrêa Riedel; José Augusto Carneiro; Célia Regina Carlini; Maria Apparecida Esquibel

Publisher
Springer
Year
1993
Tongue
English
Weight
901 KB
Volume
12
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

✦ Synopsis

Isolated embryos, cotyledons and embryos plusa fragment of cotyledon from seeds of Canavalia ensiformis (jack bean) were cultured in vitro. Concanavalin A and canatoxin cross-reactive material were detected by double immunodiffusion tests. Canatoxin was detectable until 30 days in cultures of embryos, embryos plus cotyledons and hypocotyls. Concanavalin A was also present in all cultures being detected until 90 days in cultures treated with 6-benzylaminopurine. No concanavalin A was detected in root cultures. Concanavalin A was present in cell suspensions until 45 days of culture; the culture medium contained neither concanavalin A nor canatoxin. Tissue cultures thus can produce Con A and CNTX and will be an important research tool for studying the biosynthesis of such substances.

📜 SIMILAR VOLUMES

The binding of fluorescent-labelled conc
The binding of fluorescent-labelled concanavalin A to human breast tissue—A marker of differentiation
✍ Rosemary A. Walker 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 682 KB

Fluorescent-labelled Concanavalin A (ConA) has been applied to frozen and fixed, processed sections of normal, hyperplastic and malignant human breast tissue. No loss of binding activity occurs with fixation and the best results are obtained with Bouin's fixed material or trypsinised sections of tis

The use of pronase in tissue culture: A
The use of pronase in tissue culture: A comparison with trypsin
✍ John F. Foley; Byron Aftonomos 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 English ⚖ 340 KB

## Abstract Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and complete dispersing agent for primary fibroblastic cell lines and clearly superior to trypsin. On the other hand, with certain continuous