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The use of pronase in tissue culture: A comparison with trypsin

✍ Scribed by John F. Foley; Byron Aftonomos

Publisher
John Wiley and Sons
Year
1970
Tongue
English
Weight
340 KB
Volume
75
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Pronase is a proteolytic enzyme recently introduced as an effective dispersing agent of tissue culture cells. We have found it to be a most rapid and complete dispersing agent for primary fibroblastic cell lines and clearly superior to trypsin. On the other hand, with certain continuous epithelial cell lines its completeness of dispersion is inferior to that of trypsin necessitating study of each cell line individually with both enzymes to select the most advantageous one.

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