𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The preparation of immunoadsorbents and their adsorption properties for anti-DNA antibodies in SLE serum

✍ Scribed by Yi-hua Yu; Bing-lin He

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
172 KB
Volume
41
Category
Article
ISSN
1381-5148

No coin nor oath required. For personal study only.

✦ Synopsis

By using the hydroxyethyl crosslinked chitosan beads as the support, a novel immunoadsorbent was prepared by conjugating DNA onto the beads via the CNBr activation. The influence of conditions on the immobilization capacity of DNA was studied in detail. The adsorption experiments were performed by adding the immunoadsorbents to the SLE serum. The results showed that this type of immunoadsorbent could cut down the anti-DNA antibody levels by 65.33% at most in serum. Because the chitosan has good blood compatibility, this type of immunoadsorbent is hopeful for the treatment of SLE by hemoperfusion in the future.

📜 SIMILAR VOLUMES

Myth and reality: practical test system
Myth and reality: practical test system for the measurement of anti-DNA antibodies in the diagnosis of systemic lupus erythematosus (SLE)
✍ Laura J. McCloskey; Paul Christner; Dana Jacobs-Kosmin; Troy D. Jaskowski; Harry 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB

## Abstract The myth persists that only the labor intensive Farr radioimmunoassay and __Crithidia luciliae__ immunofluorescence (CL‐IFA) are systemic lupus erythematosus (SLE)‐specific tests. We compared them to ELISA with bacteriophage λ DNA (EL‐dsDNA) and denatured calf thymus DNA (EL‐ssDNA). By

Detection of cross-reactive anti-DNA ant
Detection of cross-reactive anti-DNA antibody idiotypes in the serum of systemic lupus erythematosus patients and of their relatives
✍ Dr. D. A. Isenberg; Y. Shoenfeld; M. Walport; C. Mackworth-Young; C. Dudeney; A. 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 757 KB

Two common cross-reacting anti-DNA antibody idiotypes designated 1616 and 32/15, previously identified in the serum of patients who have systemic lupus erythematosus, were found in 24% and 7%, respectively, of 147 first-degree relatives. These findings imply that high-frequency germ-line genes exist