Density gradients of sucrose can be prepared in large numbers by successive freezing and thawing of sucrose solutions. Gradients of other solute molecules, such as salt and detergents, also form and this could affect subsequent sedimentation behavior of some molecules. However, the sedimentation beh
The preparation of freeze-thaw density gradients with homogeneous solute concentrations
โ Scribed by A.J. Cooper; J.A. Smallwood; R.A. Morgan
- Book ID
- 118899311
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 323 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0022-1759
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๐ SIMILAR VOLUMES
Phospholipid vesicles prepared by the freeze-thaw extrusion method contain internal solute concentrations which are much higher than the external values (entrapment ratios much greater than 1). This concentrating effect is a complex function of the total impermeant solute concentration in the medium
## One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles In the present study, we describe a new procedure using freezing-thawing t