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The pattern of density dependent growth inhibition in murine fibroblasts

✍ Scribed by Mary C. P. Canagaratna; P. A. Riley

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 842 KB
Volume: 85
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040850214

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✦ Synopsis

Abstract

Observations on the pattern of nuclear incorporation of ^3^H‐TdR in long term (8‐day) and short term (3‐day) 3T3 cultures with local cell densities between 0.2 × 10^4^ and 6.2 × 10^4^ cells/cm^2^ are reported. Contrary to a number of previous studies our observations indicate that density dependent inhibition is exhibited in relatively sparse cultures, commencing at 0.5 × 10^4^ cells/cm^2^. Various possible mechanisms which could have caused the observed pattern of density‐dependent regression in labelling index are discussed.

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