Density-dependent effects of oxygen on the growth of mammalian fibroblasts in culture

✍ Scribed by Dr. William G. Taylor; Richard F. Camalier; Katherine K. Sanford

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 776 KB
• Volume: 95
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040950105

✦ Synopsis

Abstract

Various concentrations of oxygen were used to determine the optimum culture medium P for survival and proliferation of attached human and mouse fibroblasts grown from different inoculum sizes. When T−15 flasks were seeded with ⪕ 2 × 10^4^ cells (⪕ 1.3 × 10^3^ cells/cm^2^), the highest plating efficiencies and cell yields were obtained with a culture medium P of 40—60 mm Hg. At higher inoculum sizes (10^5^ cells per T‐15) used routinely for mass cultures, no difference in cell yield or glycolytic activity was observed between cultures gassed with atmospheric, i.e., 18% O~2~ (growth medium P ≅ 125—135 mm Hg) and those gassed with 1% O~2~ (growth medium P ≅ 40—60 mm Hg). The enhanced clonal growth observed at the latter P results from an increased proliferation rate rather than more efficient attachment and survival of inoculated cells. Glucose uptake and lactic acid accumulation were increased in sparse cultures sparged with 1% O~2~. A slight extension of lifespan was observed in WI−38 cells serially subcultured with a gas phase of 1% O~2~.