The oxidation and assimilation of acetate by bakers' yeast
โ Scribed by Winzler, Richard J.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1940
- Tongue
- English
- Weight
- 623 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0095-9898
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โฆ Synopsis
In most studies on the aerobic exogenous metabolism of tissues and microorganisms, attention has been focused upon the oxidation of the substrate to carbon dioxide and water. When non-proliferating or resting cells have been used in short term experiments, it has generally been assumed that these oxidations were the only reactions which were quantitatively significant, and that syntheses were negligible.
It has been shown by Barker ( '36), Giesberger ( '36), and Clifton ( '37) that when acetate is added to resting bacterial cultures a considerable portion may be converted to cell material. From indirect evidence they concluded that the synthesized material was a Carbohydrate. This paper gives the results of an investigation of the respiration of yeast in the presence of acetate, the synthesis of carbohydrate from acetate by yeast, and the influence of various conditions upon the extent of acetate oxidation and assimilation.
๐ SIMILAR VOLUMES
The gradual recognition that carbon sources play a dual role in cell metabolism, donating electrons for cell reductions and molecules for cell syntheses led to experiments of Barker ('36) and others (Doudoroff, '40; Winder, '40; Clifton, '46; Syrett, '51; Blumenthal et al., '57; Wilson and Danforth,
This research wau supported in part by grants from the Rockefeller Foundation and the Wisconsin Alumni Research Foundation. 'We have not used NaN, extensively, since it is not as effective as DNP in inhibiting assimilation by the rhizobia, and since it is B powerful inhibitor of their respiration (B
## Abstract The rates of release of 7 enzymes from bakers' yeast have been measured. The disruption process did not cause loss of activity of these enzymes. The various operating pressures, temperatures, and initial yeast concentrations used did not affect the rates of enzyme release relative to pr