𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The origin of Q-independent derivatives of phage λ

✍ Scribed by Kaiser, Kim

Book ID
104695755
Publisher
Springer
Year
1980
Tongue
English
Weight
698 KB
Volume
179
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

lambda qsr' (Q-independent) phages are characterised by the replacement of the region of the lambda genome that contains Q, S, R, and the late gene promoter, P'R, with host-derived DNA that codes for functions analogous to those deleted. Restriction endonuclease analysis and DNA/DNA hybridisation methods have been used to show that lambda p4 and lambda qin A3, two such Q-independent phages, are the product of recombination between lambda and a defective lambdoid prophage (the qsr' prophage) located at an as yet unidentified site in the E. coli K 12 chromosome. The qsr' prophage is distinct from the defective lambdoid prophage Rac (Kaiser and Murray 1979). In the E. coli K12 strain AB1157 from which lambda qsr' phages cannot be generated, the qsr' prophage has suffered an internal deletion. That the qsr' prophage appears not to carry a full complement of essential late genes suggests one explanation for its apparently defective nature.

📜 SIMILAR VOLUMES

The ral gene of phage λ
The ral gene of phage λ
✍ Debrouwere, Lieve ;Zabeau, Marc ;Van Montagu, Marc ;Schell, Jozef 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 646 KB

The lambda ral function modulates the restriction and modification activities of the Escherichia coli K12 and B restriction enzymes (Zabeau et al., 1980). In order to further analyse this function, ral deficient mutants have been isolated, using a method which exploits the property of the strong mut