Specific Binding of the λ Phage Repressor to λ DNA
✍ Scribed by PTASHNE, MARK
- Book ID
- 109656488
- Publisher
- Nature Publishing Group
- Year
- 1967
- Tongue
- English
- Weight
- 305 KB
- Volume
- 214
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/214232a0
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Phage λ Orf substitutes for the activities of the __Escherichia coli__ RecFOR proteins __in vivo__ and is therefore implicated as a recombination mediator, encouraging the assembly of bacterial RecA onto single‐stranded DNA (ssDNA) coated with SSB. Orf exists as a dimer in solution, ass
A method has been developed for radiolabeling the lambda cl repressor to a specific activity sufficiently high to permit accurate quantitation of the protein in the picomolar range of concentration. Procedures are described whereby the labeled protein can be used for accurate quantitative study of t