The oral apparatus ofTetrahymena pyriformis, strain WH-6. III. The binding of3H-cytochalasin B by the isolated oral apparatus

✍ Scribed by Gavin, R. H.

• Book ID: 102336189
• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 353 KB
• Volume: 197
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401970107

✦ Synopsis

Abstract

The binding of tritium‐labelled cytochalasin B by the isolated oral apparatus of Tetrahymena pyriformis, strain WH‐6, syngen 1, was investigated. Equilibrium binding studies revealed approximately 1.4 × 10^5^ cytochalasin B binding sites per oral apparatus. A Scatchard plot indicates a single class of binding affinities with an association constant of 10^5^ liters/mole. Rapid release of oral apparatus‐bound cytochalasin B occurred when oral apparatuses were washed and resuspended in 1 mM TRIS without cytochalasin B. Because cytochalasin B binding to oral apparatus microtubular protein was not detected, microtubules are probably not the cytochalasin B binding site. The probable nature of the cytochalasin B binding site within oral apparatus is discussed.