The oral apparatus ofTetrahymena pyriformis, Strain WH-6. II. Cytochalasin B inhibition of oral apparatus morphogenesis

✍ Scribed by Gavin, R. H.

• Book ID: 102336188
• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 471 KB
• Volume: 197
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401970106

✦ Synopsis

Abstract

The effects of cytochalasin B on oral apparatus morphogenesis and cell division were studied in synchronized Tetrahymena pyriformis, strain WH‐6, syngen 1. Cytochalasin B brought about the rapid arrest of oral apparatus primordium development when added prior to the completion of oral apparatus membranelle differentiation. Cells arrested in development did not divide. When cytochalasin B was added after this transition point, oral apparatus morphogenesis and cell division were completed. The effects of cytochalasin B could be reversed by washing it from the medium. Even though cytochalasin B (at 400 μg/ml) reduced protein synthesis by 30%, the data are consistent with the interpretation that cytochalasin B prevents an assembly process during the membranelle differentiation phase of oral apparatus development.