The nusG gene of Streptomyces griseus: Cloning of the gene and analysis of the A-factor binding properties of the gene product
✍ Scribed by Sabine Kuberski; Thomas Kasberg; Jürgen Distler
- Book ID
- 109319985
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 516 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0378-1097
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A central segment of a cluster of biosynthetic genes for the antibiotic streptomycin cloned from Streptomyces griseus was analysed for open reading frames, as well as for transcriptional and translational activity. The nucleotide sequence revealed two significant open reading frames, ORF1 and APH(6)
A 3 kb genomic fragment containing the nusG gene of Streptomyces coelicolor A3(2) was identified, cloned and sequenced. Sequence analysis revealed 3 complete and 2 truncated open reading frames (ORFs): truncated ORFu (similar to a Bacillus gene encoding a thermostable aspartate aminotransferase)-sec