Cloning, nucleotide sequence, and transcriptional analysis of the nusG gene of Streptomyces coelicolor A3(2), which encodes a putative transcriptional antiterminator

✍ Scribed by Puttikhunt, Chunya ;Nihira, Takuya ;Yamada, Yasuhiro

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 627 KB
• Volume: 247
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425829

✦ Synopsis

A 3 kb genomic fragment containing the nusG gene of Streptomyces coelicolor A3(2) was identified, cloned and sequenced. Sequence analysis revealed 3 complete and 2 truncated open reading frames (ORFs): truncated ORFu (similar to a Bacillus gene encoding a thermostable aspartate aminotransferase)-secE (94 amino acids; 79.0% similarity to Escherichia coli SecE)-nusG (300 amino acids; 73.3% similarity to E. coIi NusG)-rplK (144 amino acids; 88.5% similarity to E. coli ribosomal subunit L l l ) -t r u n c a t e d rplA (similar to E. coli ribosomal subunit L1). The gene organization secE-nusG-rpIKA exactly matches that in E. coli. Transcriptional analyses by the primer extension method revealed one transcriptional start site each for secE and nusG, and two sites for rplK. The presence of promoters was also confirmed with the aid of a promoter-probe vector.