The nucleotide sequence and evolution of ovine MHC class IIBgenes:DQBandDRB

✍ Scribed by Peter C. Scott; Jillian F. Maddox; Kathryn J. Gogolin-Ewens; Malcolm R. Brandon

• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 707 KB
• Volume: 34
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00211420

✦ Synopsis

The nucleotide sequences of one Ovar-DQB gene, excluding exon 1 and parts of the introns, and one Ovar-DRB pseudogene are presented. The structure of the Ovar-DQB gene is typical of a major histocompatibility complex (MHC) class II B gene and demonstrates considerable sequence similarity with that of humans including such characteristics as the less common polyadenylation signal, ATTAAA. The ovine sequence has a typical 5' acceptor splice signal for exon 5, thus potentially encoding a full length cytoplasmic tail. The Ovar-DRB gene identified in this study was found to be a pseudogene, lacking a defined exon 2 and containing premature termination codons in both exons 3 and 4. The 3' donor splice site of exon 3 is also atypical. A purine-pyrimidine microsatellite repeat, (dC.dA)15, in the 3' region of the pseudogene may be a hotspot for recombination within the ovine DR subregion.