The nuclear role in the ultraviolet inactivation of Neurospora conidia

✍ Scribed by Norman, Amos

• Publisher: Wiley (John Wiley & Sons)
• Year: 1954
• Tongue: English
• Weight: 455 KB
• Volume: 44
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030440102

✦ Synopsis

An analysis of the survival curves of ultraviolet irradiated Keurospora conidia indicates that the inactivation of tlie conidia is a consequence of the inactivation of the nuclei (Norman, '51). The inactivation cross section per nucleus is about twice as large in uninucleate (haploid) conidia as in niultinucleate conidia ; the smaller cross section per nucleus in the inultiiiucleate conidia is apparently clue to some sort of nuclear interaction. The present study of the influence of nuclcar number on the inactivation and photoreactivation of Keurospora conidia was undertaken to establish more firinly tlie role of the nucleus in the inactivation process, and to learn sonietl~ing inorc of the nature of the nuclear interaction in multinucleate conidia.

31 E I ~A L S AXL) METHODS

Neurosporn crns.w 19, which gives niultinucleatc macroconidia, and the mutant strain pe"'F1, which gives uninucleate niicroconidia wcre grown 011 minimal medium (Norman, 51). On this medium the macrocoiiitlia average 2.3 nuclei per conidiuni. This number is increased to 4.2 and 5.9 by adding,