✦ LIBER ✦

Nuclear suppressors of the [poky] cytoplasmic mutant in Neurospora crassa

✍ Scribed by Collins, Richard A. ;Bertrand, Helmut

Publisher: Springer
Year: 1978
Tongue: English
Weight: 582 KB
Volume: 161
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00331000

No coin nor oath required. For personal study only.

✦ Synopsis

We have previously isolated six non-allelic, nuclear mutations (sui loci) that partially suppress the growth, respiratory and cytochrome abnormalities of the extranuclear [poky] mutant. A comparison of the mitochondrial ribosome profiles of suppressed and unsuppressed [poky] strains revealed that five of the six suppressors alleviate at least partially the deficiency of mitochondrial small ribosomal subunits that is associated with the [poky] genotype. Six independently isolated Group 1 extranuclear mutants, namely [exn-1], [exn-2], [exn-4-a1, [stp-b1], [SG-1] and [SG-3-A1, which have growth and cytochrome phenotypes similar to [poky] also were found to be deficient in small subunits of mitochondrial ribosomes. Using cytochrome aa3 and b production as a criterion for mitochondrial protein synthesis, it could be shown that the nuclear su I suppressors of [poky] also suppress the other six Group I extranuclear mutants. However, differences in the efficiencies of suppression by suI suppressors suggest that at least some of Group I extrachromosomal mutants are not simply re-isolates of [poky], but represent distinct extranuclear mutations.

📜 SIMILAR VOLUMES

The regulation of nitrate assimilation i

The regulation of nitrate assimilation in Neurospora crassa: Biochemical analysis of the nmr-1 mutants

✍ Dunn-Coleman, Nigel S. ;Tomsett, A. Brian ;Garrett, Reginald H. 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 567 KB

Neurospora crassa nmr-1 mutants, selected on the basis of their sensitivity to chlorate in the presence of glutamine, have elevated levels of the nitrate assimilation enzymes, NADPH-nitrate reductase and NAD(P)H-nitrite reductase. Immunoelectrophoretic determinations show that the higher nitrate red

The regulation of nitrate assimilation i

The regulation of nitrate assimilation in Neurospora crassa: The isolation and genetic analysis of nmr-1 mutants

✍ Tomsett, A. Brian ;Dunn-Coleman, Nigel S. ;Garrett, Reginald H. 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 516 KB

Four mutants of Neurospora crassa have been isolated which have altered regulation of nitrate reductase. They each carry a mutation which results in derepressed synthesis of nitrate reductase even in the presence of glutamine. They map to a single locus which has been designated nmr-1 and which is l

A novel phenotype of an excision-repair

A novel phenotype of an excision-repair mutant in Neurospora crassa: Mutagen sensitivity of the mus-18 mutant is specific to UV

✍ Ishii, Chizu ;Nakamura, Kiyoshi ;Inoue, Hirokazu 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 871 KB

A UV-sensitive mutant has been isolated from UV-mutagenized conidia of Neurospora crassa. The mutation responsible for the lesion was mapped in linkage group VL, proximal to the nucleolus organizer region. We designated the mutant mus-18. The sensitivity of the mus-18 mutant to UV-irradiation was no

Fast induction of translatable mRNA by b

Fast induction of translatable mRNA by blue light in neurospora crassa wt: The wc-1 and wc-2 mutants are blind

✍ Christiane Nawrath; V.E.A. Russot 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 966 KB

After blue-light irradiation of Neurospora crassa (wt) mycelia we observed an increase of about 13 translatable mRNA species within a period of 30 min. The induction of translatable mRNA species followed a specific temporal pattern which permitted the identification of four distinct classes. One of

Light-induced Dephosphorylation of a 33

Light-induced Dephosphorylation of a 33 kDa protein in the wild-type strain of Neurospora crassa: The regulatory mutants wc-i and wc-2 are abnormal

✍ Frank-R. Lauter; Vincenzo E.A. Russo 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 625 KB

Light induces the dephosphorylation of a 33 kdalton protein within 8 min in the wild-type strain of Neurospora crassa. The regulatory mutants, wc-1 and wc-2, have an altered pattern of phosphoproteins in darkness and also after irradiation. Because the wc genes have previously been implicated in pho

A cytoplasmic gene for partial suppressi

A cytoplasmic gene for partial suppression of a nuclear pleiotropic respiratory deficient mutant in the petite negative yeast Schizosaccharomyces pombe

✍ Colson, Anne-Marie ;Labaille, Françoise ;Goffean, André 📂 Article 📅 1976 🏛 Springer 🌐 English ⚖ 863 KB

The nuclear pleiotropic respiratory-deficient mutant pet1 (previously M126) exhibits cytochromes aa3 and b deficiencies accompanied by loss of the oligomycin-sensitivity of the mitochondrial ATPase. The mutant pet1, unable to grow on glycerol, growth on glucose. The latter phenotypic trait symbolize