The new paradigm: Integrating genomic function and nuclear architecture

✍ Scribed by Ronald Berezney; Xiangyun Wei

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 159 KB
• Volume: 72
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/(sici)1097-4644(1998)72:30/31+<238::aid-jcb29>3.0.co;2-f

✦ Synopsis

A new view of the cell nucleus is emerging based on the functional dynamics of nuclear architecture. The striking structural preservation of a variety of genomic processes on the nuclear matrix provides an important approach for correlating nuclear form and function. In situ labeling coupled with three-dimensional microscopy and computer imaging techniques shows that DNA replication and transcription sites are organized into higher-order units, or ''zones,'' in the cell nucleus. The dynamic interplay and ''re-zoning'' of replication and transcription regions during the cell cycle may form the structural basis for the elaborate global coordination of replicational and transcriptional programs in the mammalian cell.